7D6Y
eIF4E in Complex with a Disulphide-Free Autonomous VH Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953700 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.848, 45.324, 39.763 |
| Unit cell angles | 90.00, 93.19, 90.00 |
Refinement procedure
| Resolution | 44.226 - 1.668 |
| Rwork | 0.181 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8w |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.197 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.229 | 1.760 |
| High resolution limit [Å] | 1.668 | 1.670 |
| Rmerge | 0.116 | 0.673 |
| Number of reflections | 41261 | 5689 |
| <I/σ(I)> | 6.1 | 1.1 |
| Completeness [%] | 99.4 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 25% (w/v) PEG 6000, 0.01-02 M Ammonium Sulphate, 100mM Tris, pH 8.5 |
