7D6F

The crystal structure of ARMS-PBM/MAGI2-PDZ4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97776
Spacegroup name	C 2 2 2
Unit cell lengths	74.045, 95.666, 35.167
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.277 - 3.001
R-factor	0.2381
Rwork	0.235
R-free	0.28140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zys
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (3.25)
Refinement software	PHENIX (v1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.092	0.047	0.724
Rmeas	0.096	0.049	0.757
Rpim	0.027	0.014	0.219
Total number of observations	46340
Number of reflections	3696	218	178
<I/σ(I)>	4.9
Completeness [%]	100.0	99.5	100
Redundancy	12.5	10.5	11.8
CC(1/2)		0.999	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 8000, 8% v/v Ethylene glycol