7D57
C-Src in complex with FIIN-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-05-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.798, 62.964, 74.138 |
| Unit cell angles | 78.98, 89.78, 89.69 |
Refinement procedure
| Resolution | 27.405 - 2.104 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u5j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.867 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.405 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 35316 | 4487 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 96.7 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.980 | 0.680 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 2% glycerol, 8% PEG 4000, 50mM sodium acetate, 10mM MgCl2 |
