7CZM

Crystal structure of FIP200 Claw/p-OPtineurin LIR complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	73.978, 70.624, 56.337
Unit cell angles	90.00, 109.27, 90.00

Refinement procedure

Resolution	34.960 - 2.000
Rwork	0.203
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dce
RMSD bond length	0.007
RMSD bond angle	0.865
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.320	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.034	0.254
Number of reflections	18560	1836
<I/σ(I)>	31.6
Completeness [%]	99.6
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Potassium iodide, MES pH 6.5, PEG 4000