7CYU

Crystal structure of human BAF57 HMG domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2019-03-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	58.873, 58.873, 50.263
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.790 - 2.550
R-factor	0.2377
Rwork	0.233
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tq6
RMSD bond length	0.003
RMSD bond angle	0.471
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.550	2.550
Number of reflections	3481	312
<I/σ(I)>	21.62	1.88
Completeness [%]	98.9	95.2
Redundancy	7
CC(1/2)	0.998	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	290	2.0M Ammonium sulfate, 0.1M Sodium acetate