7CYM
Crystal structure of LI-Cadherin EC1-4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.360, 70.840, 134.219 |
Unit cell angles | 90.00, 98.69, 90.00 |
Refinement procedure
Resolution | 55.250 - 2.700 |
R-factor | 0.2247 |
Rwork | 0.222 |
R-free | 0.27440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zmy |
RMSD bond length | 0.013 |
RMSD bond angle | 1.835 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.200 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.095 | 0.858 |
Rpim | 0.041 | 0.371 |
Number of reflections | 41272 | 5955 |
<I/σ(I)> | 1.8 | 1.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.1 | 6.2 |
CC(1/2) | 0.998 | 0.907 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 200 mM sodium sulfate, 20% w/v Polyethylene glycol 3350 |