7CWE

Human Fructose-1,6-bisphosphatase 1 in APO R-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-26
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97853
Spacegroup name	P 41 2 2
Unit cell lengths	125.305, 125.305, 108.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.960 - 3.000
R-factor	0.2614
Rwork	0.258
R-free	0.29630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vt5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.960	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.197
Number of reflections	17241	1627
<I/σ(I)>	8.22
Completeness [%]	97.0
Redundancy	5.62
CC(1/2)	0.989	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.05 M cadmium sulfate, 0.1 M HEPES, pH 7.5, 1 M sodium acetate