7CVH
Human Fructose-1,6-bisphosphatase 1 in complex with geranylgeranyl diphosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-01 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.392, 83.426, 277.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.250 - 2.090 |
R-factor | 0.1789 |
Rwork | 0.178 |
R-free | 0.21830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vt5 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.090 | 5.700 | 2.100 |
Rmerge | 0.366 | 0.078 | 1.019 |
Rmeas | 0.413 | 0.088 | 1.240 |
Rpim | 0.187 | 0.040 | 0.692 |
Number of reflections | 89144 | 4767 | 4257 |
<I/σ(I)> | 3.9 | ||
Completeness [%] | 95.8 | 95 | 93.5 |
Redundancy | 3.7 | 3.8 | 2.5 |
CC(1/2) | 0.997 | 0.352 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 28% (v/v) polyethylene glycol 600, 0.1M HEPES |