7COD
Post insertion complex of DNA polymerase Mu (K438A/Q441A) with 1-nt gapped DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2019-12-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.180, 68.520, 111.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.773 - 1.800 |
R-factor | 0.1868 |
Rwork | 0.186 |
R-free | 0.21090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.773 | 29.773 | 1.850 |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.036 | 0.017 | 0.303 |
Rmeas | 0.040 | 0.020 | 0.383 |
Total number of observations | 195058 | ||
Number of reflections | 41495 | 546 | 2264 |
<I/σ(I)> | 24.19 | 60.16 | 2.55 |
Completeness [%] | 95.1 | 96.1 | 71.5 |
Redundancy | 4.701 | 4.359 | 2.223 |
CC(1/2) | 1.000 | 0.999 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 298 | 1,4-Dioxane, Tris-Hcl, PEG 3350 |