7COB
Ternary complex of DNA polymerase Mu (Q441A) with 1-nt gapped DNA (T:dGMPNPP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2019-10-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.760, 68.340, 110.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.663 - 1.797 |
R-factor | 0.1809 |
Rwork | 0.180 |
R-free | 0.20380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.663 | 29.663 | 1.840 |
High resolution limit [Å] | 1.797 | 8.040 | 1.800 |
Rmerge | 0.049 | 0.026 | 0.576 |
Rmeas | 0.055 | 0.030 | 0.637 |
Number of reflections | 42776 | 539 | 3037 |
<I/σ(I)> | 16.91 | 38.7 | 2.63 |
Completeness [%] | 99.7 | 96.2 | 97.1 |
Redundancy | 5.448 | 4.525 | 5.465 |
CC(1/2) | 0.999 | 0.999 | 0.874 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 298 | 1,4-Dioxane, Tris-HCl, PEG 3350 |