7CO5

HtrA-type protease AlgW with decapeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	95.120, 130.837, 250.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.500 - 2.345
R-factor	0.2684
Rwork	0.268
R-free	0.28920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1soz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.500	2.429
High resolution limit [Å]	2.345	2.345
Number of reflections	213255	11425
<I/σ(I)>	2.96
Completeness [%]	96.1
Redundancy	3.4
CC(1/2)	0.796	0.262

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	2% Tacsimate PH 7.0, 5% 2-Propanol, 0.1M Imidazole PH 7.0, 8% PEG3350