7CNL

Crystal structure of TEAD3 in complex with VT105

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	150.808, 65.372, 123.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.371 - 2.600
Rwork	0.224
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5emw
RMSD bond length	0.003
RMSD bond angle	1.238
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	150.810	2.720
High resolution limit [Å]	2.600	2.600
Number of reflections	38106	4548
<I/σ(I)>	15.1
Completeness [%]	99.7
Redundancy	11.6
CC(1/2)	0.999	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	1.26M Sodium di-hydrogen Phosphate, 0.14M di-Potassium hydrogen Phosphate, PH 5.6