7CM4

Crystal Structure of COVID-19 virus spike receptor-binding domain complexed with a neutralizing antibody CT-P59

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9796
Spacegroup name	I 2 2 2
Unit cell lengths	65.550, 167.600, 169.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.760 - 2.710
R-factor	0.2186
Rwork	0.217
R-free	0.24180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg
RMSD bond length	0.003
RMSD bond angle	0.588
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.18.2_3874)
Refinement software	Coot (0.8.9.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.760	2.807
High resolution limit [Å]	2.710	2.710
Rmeas	0.254	2.232
Rpim	0.069	0.613
Number of reflections	25744	2541
<I/σ(I)>	12.44	1.63
Completeness [%]	99.8
Redundancy	13.6	99.88
CC(1/2)	0.997	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	100 mM Tris-Cl pH 8.0 16% (w/v) Polyethylene glycol monomethyl ether 2,000 10 mM Nickel(II) Chloride hexahydrate