7CLY

Structure of the DOCK8 DHR-1 domain crystallized with di-C8-phosphatidylinositol-(4,5)-bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-06
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	89.046, 89.046, 49.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.084 - 1.432
R-factor	0.1957
Rwork	0.195
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3clx
RMSD bond length	0.005
RMSD bond angle	0.819
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.430	1.430
Rmeas	0.070	0.963
Number of reflections	37170	3588
<I/σ(I)>	37	3.3
Completeness [%]	99.8	98.4
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	PEG 3350, sodium sulfate decahydrate