7CJB
VHL recognizes hydroxyproline in RIPK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 94.831, 94.831, 361.605 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.060 - 2.800 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ajy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.679 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
| Rmerge | 0.137 | 0.053 | 1.766 |
| Rmeas | 0.146 | 0.056 | 1.881 |
| Rpim | 0.048 | 0.016 | 0.625 |
| Total number of observations | 609253 | ||
| Number of reflections | 34589 | 3972 | 3764 |
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 95.3 | 99.9 | 97.8 |
| Redundancy | 8.2 | 12 | 8.4 |
| CC(1/2) | 0.999 | 0.544 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 289 | sodium cacodylate pH 6.5, 100 mM magnesium acetate, 15%(w/v) PEG 6000 |
