7CJB

VHL recognizes hydroxyproline in RIPK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97949
Spacegroup name	P 41 2 2
Unit cell lengths	94.831, 94.831, 361.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.060 - 2.800
R-factor	0.2219
Rwork	0.220
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ajy
RMSD bond length	0.008
RMSD bond angle	1.679
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.137	0.053	1.766
Rmeas	0.146	0.056	1.881
Rpim	0.048	0.016	0.625
Total number of observations	609253
Number of reflections	34589	3972	3764
<I/σ(I)>	6.7
Completeness [%]	95.3	99.9	97.8
Redundancy	8.2	12	8.4
CC(1/2)		0.999	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	289	sodium cacodylate pH 6.5, 100 mM magnesium acetate, 15%(w/v) PEG 6000