7CII
Crystal structure of L-methionine decarboxylase from Streptomyces sp.590 in complexed with L- methionine methyl ester (external aldimine form).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.322, 147.588, 54.011 |
| Unit cell angles | 90.00, 99.64, 90.00 |
Refinement procedure
| Resolution | 19.910 - 1.510 |
| R-factor | 0.17534 |
| Rwork | 0.174 |
| R-free | 0.19567 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cif |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.456 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.094 | 0.714 |
| Rpim | 0.117 | 0.885 |
| Number of reflections | 143471 | 46587 |
| <I/σ(I)> | 6.5 | 1.3 |
| Completeness [%] | 97.9 | 96 |
| Redundancy | 2.6 | |
| CC(1/2) | 0.992 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M MMT buffer pH 6.0, 23.5 % (w/v) PEG1500 |
