7CCC
The structure of the actin filament uncapping complex mediated by twinfilin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-07 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.759, 168.662, 176.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 3.050 |
High resolution limit [Å] | 3.000 | 7.980 | 3.000 |
Rmerge | 0.188 | 0.067 | 2.080 |
Rmeas | 0.198 | 0.070 | 2.224 |
Rpim | 0.061 | 0.021 | 0.761 |
Total number of observations | 403423 | ||
Number of reflections | 34562 | 2428 | 2097 |
<I/σ(I)> | 4.5 | ||
Completeness [%] | 95.7 | 99.1 | 94.4 |
Redundancy | 9.3 | 9.6 | 7.2 |
CC(1/2) | 0.997 | 0.495 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 12% PEG 8000, 10% glycerol, 500 mM potassium chloride |