7CBE

Crystal structure of Homoserine O-succinyltransferase from Escherichia coli K-12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	76.348, 81.027, 98.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.750 - 1.700
R-factor	0.226
Rwork	0.225
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h2w
RMSD bond length	0.010
RMSD bond angle	1.547
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.075	0.056	0.282
Rmeas	0.081	0.061	0.324
Rpim	0.031	0.023	0.154
Total number of observations	342170
Number of reflections	65291	3494	3176
<I/σ(I)>	12.7
Completeness [%]	97.0	96.3	95.8
Redundancy	5.2	6.7	3.7
CC(1/2)		0.996	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	283	25% Polyethylene glycol 3350, 0.1M Bis-tris pH 5.5, 0.2M Ammonium sulfate