7C5N
Crystal Structure of C150A+H177A mutant of Glyceraldehyde-3-phosphate-dehydrogenase1 from Escherichia coli at 2.0 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97776 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.376, 89.376, 341.506 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.990 |
R-factor | 0.16391 |
Rwork | 0.161 |
R-free | 0.21896 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7c5f |
RMSD bond length | 0.019 |
RMSD bond angle | 1.937 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.020 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.200 | 0.880 |
Number of reflections | 96080 | 96080 |
<I/σ(I)> | 18.55 | 5.25 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.5 | 18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 100mM sodium Cacodylate pH 6.3, 20%(w/v) PEG 1000, 200mM MgCl2 |