7C43
The crystal structure of Trypanosoma brucei RNase D : AMP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-06-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.730, 77.730, 99.301 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.210 - 2.300 |
R-factor | 0.2021 |
Rwork | 0.200 |
R-free | 0.24240 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL2Map |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 61.210 | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.071 | 0.053 | 0.279 |
Rmeas | 0.074 | 0.056 | 0.307 |
Rpim | 0.023 | 0.016 | 0.122 |
Number of reflections | 17294 | 1899 | 1594 |
<I/σ(I)> | 8.9 | ||
Completeness [%] | 97.4 | 99 | 91.6 |
Redundancy | 8.4 | 11.2 | 4.8 |
CC(1/2) | 0.998 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | 0.1 M Bis-Tris pH 5.5, 30% PEG 3350 |