7C40

MgGDP bound KRAS G12V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 63
Unit cell lengths	82.547, 82.547, 40.804
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.017 - 2.516
R-factor	0.177
Rwork	0.174
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tq9
RMSD bond length	0.009
RMSD bond angle	1.077
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.169
High resolution limit [Å]	2.500	2.516
Rmerge	0.128	0.515
Number of reflections	5543	5527
<I/σ(I)>	27.2
Completeness [%]	99.9
Redundancy	21.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	Polyethylene glycol 3,350, 0.2M potassium nitrate (pH 6.8)