7C06
Crystal structure of yeast U2AF1 complex bound to 3' splice site RNA, 5'-UAGGU.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.823, 255.101, 94.112 |
| Unit cell angles | 90.00, 101.13, 90.00 |
Refinement procedure
| Resolution | 38.410 - 3.020 |
| R-factor | 0.2162 |
| Rwork | 0.215 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yh8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.511 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.860 | 3.080 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.113 | 1.040 |
| Number of reflections | 84760 | 4562 |
| <I/σ(I)> | 17 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 6.7 |
| CC(1/2) | 0.997 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M NaCitrate (pH 6.0), 10% PEG 35000 |
