7C01

Molecular basis for a potent human neutralizing antibody targeting SARS-CoV-2 RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	87.400, 106.730, 170.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.830 - 2.880
R-factor	0.2204
Rwork	0.218
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lgz 4tsa
RMSD bond length	0.004
RMSD bond angle	0.776
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.880	2.880
Number of reflections	36863	2670
<I/σ(I)>	12.2
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.997	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	24 % w/v PEG 1500, 20 % v/v Glycerol