7BXO
Crystal structure of the toxin-antitoxin with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.510, 100.680, 132.510 |
Unit cell angles | 90.00, 96.44, 90.00 |
Refinement procedure
Resolution | 36.140 - 2.770 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yep |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.680 | 2.860 |
High resolution limit [Å] | 2.770 | 2.770 |
Number of reflections | 36307 | 2668 |
<I/σ(I)> | 15.9 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.8 | |
CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.1 M Tris-HCl , 0.2 M potassium sodium tartrate, and 12-20% (v/v) PEG 3350 |