7BVZ
Crystal structure of MreB5 of Spiroplasma citri bound to ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-03-09 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 37.950, 41.150, 56.360 |
Unit cell angles | 82.34, 74.01, 80.42 |
Refinement procedure
Resolution | 36.142 - 2.300 |
R-factor | 0.2303 |
Rwork | 0.228 |
R-free | 0.27570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4czi |
RMSD bond length | 0.002 |
RMSD bond angle | 0.513 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.400 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.120 | 0.630 |
Rpim | 0.110 | 0.520 |
Number of reflections | 12584 | 1233 |
<I/σ(I)> | 6.1 | 1.5 |
Completeness [%] | 87.9 | 86.2 |
Redundancy | 2.1 | 2.1 |
CC(1/2) | 0.980 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 0.15M Na-K phosphate. 16% PEG 3350, pH 7.8, 2mM ADP, 2mM MgCl2 |