7BVY

Crystal structure of MreB 5 of Spiroplasma citri bound to AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-01
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	I 1 2 1
Unit cell lengths	51.121, 54.083, 138.800
Unit cell angles	90.00, 98.18, 90.00

Refinement procedure

Resolution	49.838 - 2.500
R-factor	0.1931
Rwork	0.190
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bvz
RMSD bond length	0.002
RMSD bond angle	0.454
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.838	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.200	0.890
Rpim	0.120	0.540
Number of reflections	13181	1461
<I/σ(I)>	7	2.2
Completeness [%]	98.9	98.3
Redundancy	6.9	7.1
CC(1/2)	0.990	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	291	0.15M Na-K phosphate, 16% PEG 3350, pH 7.8, 2mM AMPPNP, 2mM MgCl2