7BTA

Crystal structure of Rheb D60K mutant bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-23
Detector	RAYONIX MX225HE
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	108.860, 47.800, 61.070
Unit cell angles	90.00, 112.75, 90.00

Refinement procedure

Resolution	31.852 - 2.600
R-factor	0.1936
Rwork	0.191
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xtq
RMSD bond length	0.009
RMSD bond angle	1.165
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.852	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.074	0.593
Number of reflections	9007	679
<I/σ(I)>	14.1	2.5
Completeness [%]	97.8
Redundancy	4
CC(1/2)	0.998	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	290	0.2 M NaH2PO4: 16% PEG 3350