7BOC

Crystal structure of the PRMT5 TIM barrel domain in complex with RioK1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-17
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.999859
Spacegroup name	P 31 2 1
Unit cell lengths	96.630, 96.630, 112.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.310 - 2.550
R-factor	0.2266
Rwork	0.225
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4x60
Data reduction software	XDS (VERSION Jan 31, 2020 BUILT=20200417)
Data scaling software	XSCALE (VERSION Jan 31, 2020 BUILT=20200417)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18-3855)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.310	48.310	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.191	0.066	4.289
Rmeas	0.196	0.068	4.395
Total number of observations	409271
Number of reflections	20297	266	1496
<I/σ(I)>	12.05	34.48	1.27
Completeness [%]	99.9	98.9	99.9
Redundancy	20.164	16.425	21.144
CC(1/2)	0.999	0.998	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.1M NaAcetate, 30% w/v PEG400, 0.2M CaAcetate