7BIK

Crystal structure of YTHDF2 in complex with m6Am

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	80.368, 80.368, 113.769
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.050 - 2.100
R-factor	0.1854
Rwork	0.183
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wqn
RMSD bond length	0.007
RMSD bond angle	0.895
Data reduction software	XDS (March 15, 2019)
Data scaling software	XSCALE (March 15, 2019)
Phasing software	PHASER (1.19_4080)
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.050	44.050	2.230
High resolution limit [Å]	2.100	6.240	2.100
Rmeas	0.084	0.033	0.743
Number of reflections	47916	1814	7675
<I/σ(I)>	19.73	59.53	3.24
Completeness [%]	100.0	99.7	99.7
Redundancy	6.9
CC(1/2)	0.999	0.999	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277.15	2 M ammonium sulphate, 0.1 M Sodium citrate tribasic pH 5.6