7BEA
Structure of human Programmed cell death 1 ligand 1 (PD-L1) with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 32.617, 54.616, 140.962 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.990 - 2.450 |
Rwork | 0.274 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5o45 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (v1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.990 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.138 | |
Number of reflections | 9843 | 956 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 7.1 | |
CC(1/2) | 0.997 | 0.474 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 1.2 M Sodium citrate tribasic dihydrate 0.01 M Sodium borate, pH 8.5 |