7BDJ

Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and mant-ATPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	99.612, 118.912, 187.353
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.340 - 2.590
R-factor	0.232
Rwork	0.230
R-free	0.28910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s8q
RMSD bond length	0.003
RMSD bond angle	0.641
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.590	2.590
Rmeas	0.208
Number of reflections	69284	10681
<I/σ(I)>	8.96	1
Completeness [%]	99.3
Redundancy	6.6
CC(1/2)	0.997	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350