7B7N

Human herpesvirus-8 gH/gL in complex with EphA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	72.940, 129.000, 267.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.440 - 2.690
R-factor	0.2247
Rwork	0.223
R-free	0.26490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hei 3phf
RMSD bond length	0.003
RMSD bond angle	0.534
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.440	2.830
High resolution limit [Å]	2.690	2.690
Number of reflections	35358	4540
<I/σ(I)>	7.9
Completeness [%]	99.6
Redundancy	13.6
CC(1/2)	0.998	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	0.1M Na-malonate pH 5, 14.2% PEG 3350 , 14 mM adenosine-5'-triphosphate disodium salt hydrate
1	VAPOR DIFFUSION, SITTING DROP	5	291	0.1M Na-malonate pH 5, 14.2% PEG 3350 , 14 mM adenosine-5'-triphosphate disodium salt hydrate