7B6V

Sheep Polyomavirus VP1 in complex with 5 mM Forssman antigen pentaose and 20 mM 3'-sialyllactosamine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-10-19
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00004
Spacegroup name	P 31
Unit cell lengths	130.404, 130.404, 221.221
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.846 - 1.798
Rwork	0.152
R-free	0.17700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y61
RMSD bond length	0.009
RMSD bond angle	1.721
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.850	1.910
High resolution limit [Å]	1.798	1.800
Rmeas	0.162	1.273
Number of reflections	390469	62546
<I/σ(I)>	11.02	1.51
Completeness [%]	99.8
Redundancy	9.9
CC(1/2)	0.997	0.471

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	KSCN, PEG 3350