7B6U

Sheep Polyomavirus VP1 in complex with 5 mM Forssman antigen pentaose and 20 mM 6'-sialyllactosamine

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-12-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00004
Spacegroup name	P 31
Unit cell lengths	129.645, 129.645, 220.431
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.470 - 1.901
Rwork	0.146
R-free	0.17260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y61
RMSD bond length	0.009
RMSD bond angle	1.650
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.480	2.020
High resolution limit [Å]	1.900	1.900
Rmeas	0.146	1.223
Number of reflections	325151	51994
<I/σ(I)>	9.46	1.33
Completeness [%]	99.8
Redundancy	5.3
CC(1/2)	0.996	0.441

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	KSCN, PEG 3350