7B1K
Crystal structure of phosphatidyl serine synthase (PSS) in the closed conformation with bound citrate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.999890 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 62.314, 70.758, 95.545 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.000 - 2.200 |
| R-factor | 0.2565 |
| Rwork | 0.254 |
| R-free | 0.30710 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.563 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.200 | 2.700 |
| Rmeas | 0.220 | 2.900 |
| Rpim | 0.080 | 1.070 |
| Number of reflections | 31163 | 2098 |
| <I/σ(I)> | 6.9 | 0.8 |
| Completeness [%] | 97.7 | 96.86 |
| Redundancy | 7.2 | 7.1 |
| CC(1/2) | 0.998 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 300 | Qiagens MBclass Suit and MBclass Suit II Molecular Dimension MemGold |
