7AZX

Crystal structure of the MIZ1-BTB-domain in complex with a HUWE1-derived peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-20
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 31 2 1
Unit cell lengths	69.110, 69.110, 97.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.560 - 2.250
R-factor	0.231
Rwork	0.228
R-free	0.27850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m52
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.720	2.400
High resolution limit [Å]	2.250	2.250
Rpim	0.066	0.684
Number of reflections	9023	450
<I/σ(I)>	13.8	1.5
Completeness [%]	68.0	18.8
Redundancy	34.2	27
CC(1/2)	0.999	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG 4000, sodium acetate