7AZ7

DNA polymerase sliding clamp from Escherichia coli with peptide 37 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	108.180, 67.620, 81.520
Unit cell angles	90.00, 128.09, 90.00

Refinement procedure

Resolution	53.990 - 1.650
R-factor	0.186
Rwork	0.184
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fvl
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.990	1.670
High resolution limit [Å]	1.640	1.640
Rpim	0.027	0.680
Number of reflections	39718	61
<I/σ(I)>	14.5	1.1
Completeness [%]	86.7
Redundancy	13
CC(1/2)	0.990	0.180

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.2 M Ammonium formate, pH 6.6, PEG 3350 20% (w/v)