7AYZ
Structure of the mouse 8-oxoguanine DNA Glycosylase mOGG1 in complex with activator TH10785
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9780 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.833, 81.802, 170.643 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.873 - 2.600 |
Rwork | 0.237 |
R-free | 0.28610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6g3y |
RMSD bond length | 0.003 |
RMSD bond angle | 1.227 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.300 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.082 | 0.986 |
Rmeas | 0.088 | 1.070 |
Rpim | 0.034 | 0.412 |
Number of reflections | 35633 | 4269 |
<I/σ(I)> | 18.3 | 2.2 |
Completeness [%] | 100.0 | |
Redundancy | 12.8 | |
CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 0.06 M Divalents, 0.1 M Buffer System 3 pH 8.5, 30 % v/v Precipitant Mix 2 (Morpheus screen, Molecular Dimensions) |