7AXP

Structural characterisation of WDR5:CS-VIP8 interaction in cis state 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 32 2 1
Unit cell lengths	99.240, 99.240, 98.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.765 - 2.432
R-factor	0.2703
Rwork	0.267
R-free	0.33070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iam
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.765	2.519
High resolution limit [Å]	2.432	2.432
Rmerge	0.086
Number of reflections	155702	92
<I/σ(I)>	16.5
Completeness [%]	99.7	0.998
Redundancy	13.6	12.8
CC(1/2)	0.996	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	10% (w/v) PEG20000, 20% (v/v) PEG550 MME, 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium L-tartrate, 0.02 M sodium oxamate