7AX1
Crystal structure of the human CCR4-CAF1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.850, 63.260, 113.070 |
| Unit cell angles | 90.00, 106.81, 90.00 |
Refinement procedure
| Resolution | 71.724 - 3.300 |
| R-factor | 0.2497 |
| Rwork | 0.248 |
| R-free | 0.27580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gmj 4b8c 3ngn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.502 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.740 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 15515 | 1174 |
| <I/σ(I)> | 11.8 | 0.8 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.999 | 0.290 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M MES, 0.1M MgCl2, 8% PEG6000 |
