7AUC

Crystal structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97794
Spacegroup name	P 1 21 1
Unit cell lengths	54.280, 107.690, 55.200
Unit cell angles	90.00, 109.31, 90.00

Refinement procedure

Resolution	51.230 - 1.530
R-factor	0.1901
Rwork	0.189
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3m
RMSD bond length	0.008
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.230	1.560
High resolution limit [Å]	1.530	1.530
Number of reflections	88499	8096
<I/σ(I)>	12.7	1.2
Completeness [%]	98.3	90.3
Redundancy	1.9
CC(1/2)	1.000	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	Morpheus-HT, condition A8, Molecular Dimensions 0.06 M divalents, 37.5% Buffer System 2 and 37.5% Precipitant Mix 4 Divalents = 0.3M magnesium chloride, 0.3M calcium chloride Buffer system 2 = 1M sodium HEPES, MOPS (acid) pH 7.5 75% Precipitant Mix 4 = 25% w/v MPD, 25% v/v PEG1000, 25% w/v PEG 3350