7ATU
The LIMK1 Kinase Domain Bound To LIJTF500025
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.234, 83.838, 98.029 |
| Unit cell angles | 90.00, 92.34, 90.00 |
Refinement procedure
| Resolution | 43.220 - 2.800 |
| R-factor | 0.2438 |
| Rwork | 0.240 |
| R-free | 0.31590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nxc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.222 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (7.0.053) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.970 | 2.940 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.031 | 0.348 |
| Number of reflections | 33787 | 4516 |
| <I/σ(I)> | 8.6 | 1.8 |
| Completeness [%] | 98.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M citrate pH 5.9 0.2 M NaCl 8% jeffamine M-600 0.005 M iron chloride |
