7AR4

Crystal structure of beta-catenin in complex with cyclic peptide inhibitor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979507
Spacegroup name	P 21 21 21
Unit cell lengths	71.380, 85.620, 142.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.620 - 2.600
Rwork	0.203
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jdh
RMSD bond length	0.006
RMSD bond angle	1.423
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.620	2.700
High resolution limit [Å]	2.600	2.600
Rmeas	0.171	3.000
Number of reflections	27652	2886
<I/σ(I)>	12.83	1.11
Completeness [%]	100.0	100
Redundancy	13.36	14.23
CC(1/2)	0.999	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	0.1 M HEPES, 4% PEG6000, 20 mM TRIS, 150 mM NaCl, 0.5 % Glycerol, 2 mM DTT