7AOV

Crystal Structure of a TRPM2 Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97933
Spacegroup name	P 1 21 1
Unit cell lengths	47.760, 188.128, 47.773
Unit cell angles	90.00, 102.66, 90.00

Refinement procedure

Resolution	46.612 - 2.000
R-factor	0.190409700336
Rwork	0.188
R-free	0.23118
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.927
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.620	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	53708	5374
<I/σ(I)>	10.28	1.46
Completeness [%]	97.2	96.91
Redundancy	13.9
CC(1/2)	0.996	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	Tris, BICINE, Diethylene glycol, Triethylene glycol, Tetraethylene glycol, Pentaethylene glycol, glycerol, PEG4000, Jeffamine M-600