7ANK
Crystal structure of sarcomeric protein FATZ-1 (d91-FATZ-1 construct) in complex with half dimer of alpha-actinin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.600, 56.920, 209.380 |
| Unit cell angles | 90.00, 94.56, 90.00 |
Refinement procedure
| Resolution | 46.940 - 3.204 |
| R-factor | 0.2562 |
| Rwork | 0.255 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d1e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 3.290 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.097 | 1.425 |
| Number of reflections | 26764 | 1897 |
| <I/σ(I)> | 14.1 | 1.4 |
| Completeness [%] | 98.9 | 93.9 |
| Redundancy | 6.6 | 6 |
| CC(1/2) | 0.998 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM Bis-Tris propane (pH 7.5), 100 mM sodium citrate, 10 mM zinc chloride, 12% w/w polyethylene glycol 3,350 |
