7ALX

Sav-SOD: Chimeric Streptavidin-cSOD as Host for Artificial Metalloenzymes

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999
Spacegroup name	P 42 21 2
Unit cell lengths	102.525, 102.525, 59.077
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.893 - 1.800
Rwork	0.179
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4irw
RMSD bond length	0.015
RMSD bond angle	1.973
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.893	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	29784	1719
<I/σ(I)>	18.9
Completeness [%]	100.0	100
Redundancy	24.4
CC(1/2)	0.999	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20 %w/v PEG 3350 (Precipitant) 0.2 M Na K Phos (Salt) 0.1 M BIS-TRIS prop 7.5 pH (Buffer)