7AI1

Crystal structure of human MDM2-G443T RING domain homodimer bound to UbcH5B-Ub (Crystal form 2)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	56.267, 80.687, 135.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.478 - 2.070
Rwork	0.212
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MDM2-E2-Ub
RMSD bond length	0.007
RMSD bond angle	1.434
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	135.910	2.110
High resolution limit [Å]	2.070	2.070
Number of reflections	38548	1861
<I/σ(I)>	9
Completeness [%]	100.0
Redundancy	6.4
CC(1/2)	0.997	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	0.1 M HEPES, 0.2 M NH4NO3, 20 % w/v PEG Smear Broad