7AG0

Complex between the bone morphogenetic protein 2 and its antagonist Noggin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 61 2 2
Unit cell lengths	103.310, 103.310, 170.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.427 - 3.104
R-factor	0.2345
Rwork	0.232
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m4u
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.427	3.180
High resolution limit [Å]	3.100	3.100
Number of reflections	10331	733
<I/σ(I)>	9.5	0.69
Completeness [%]	99.9	99.6
Redundancy	56.6	49.3
CC(1/2)	0.977	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	1.4 M NaKPO4