7AEO

Human ARTD2 in complex with DNA oligonucleotides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9687
Spacegroup name	I 41 2 2
Unit cell lengths	166.830, 166.830, 143.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.840 - 2.800
R-factor	0.2287
Rwork	0.227
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	F6IK and 5DSY
RMSD bond length	0.005
RMSD bond angle	0.739
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MrBUMP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.111	1.430
Rmeas	0.120	1.560
Number of reflections	24990	1835
<I/σ(I)>	9.25	1.59
Completeness [%]	98.9	99.6
Redundancy	6.8	6.7
CC(1/2)	0.990	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	Sample was crystallized in 0.1 M MES pH 6.5 and 1 M ammonium sulfate. Cryo solution was a mixture of 10% (v/v) glycerol, 10% (v/v) diethylene glycol, and 10% (v/v) 2-propanol with the crystallization conditions