7AD0
X-ray structure of Mdm2 with modified p53 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.811, 76.082, 197.299 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.320 - 2.070 |
| R-factor | 0.1895 |
| Rwork | 0.187 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tj2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.799 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 2.144 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmeas | 0.115 | 1.318 |
| Number of reflections | 43882 | 4309 |
| <I/σ(I)> | 16.98 | |
| Completeness [%] | 99.5 | |
| Redundancy | 13.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1 M Bis-Tris pH 5.5 with 0.2 M NaCl and 25% PEG 3350 |
